Posters et Publications
Le Laboratoire Associé Pôle Biologie Moléculaire fonctionne avec des Laboratoires Support reconnus pour leur implication dans le diagnostic moléculaire de la toxoplasmose et organise des études comparatives des méthodes diagnostiques (PCR, extraction, étapes pré-analytiques) concernant le diagnostic moléculaire de la Toxoplasmose.
Publications
Assessment of Droplet Digital PCR for the Detection and Absolute Quantification of Toxoplasma gondii: A Comparative Retrospective Study. Nabet C, Brossas JY, Poignon C, Bouzidi A, Paris L, Touafek F, Varlet-Marie E, Sterkers Y, Passebosc-Faure K, Dardé ML, Piarroux R, Denis JA. J Mol Diagn. 2023 Jul;25(7):467-476. doi: 10.1016/j.jmoldx.2023.03.006.
Molecular Diagnosis of Toxoplasmosis: Multicenter Evaluation of the Toxoplasma RealCycler Universal PCR Assay on 168 Characterized Human Samples. Brenier-Pinchart MP, Filisetti D, Cassaing S, Varlet-Marie E, Robert-Gangneux F, Delhaes L, Guitard J, Yéra H, Bastien P, Pelloux H, Sterkers Y. J Mol Diagn. 2022 Jun;24(6):687-696. doi: 10.1016/j.jmoldx.2022.03.009. Epub 2022 Apr 20.PMID: 35452843
Multicenter Comparative Assessment of the TIB MolBiol Toxoplasma gondii Detection Kit and Four Laboratory-Developed PCR Assays for Molecular Diagnosis of Toxoplasmosis. Brenier-Pinchart MP, Robert-Gangneux F, Accoceberry I, Pichard S, Garnaud C, Fricker-Hidalgo H, Lévêque MF, Hoarau G, Pelloux H, Bastien P, Sterkers Y, Varlet-Marie E. J Mol Diagn. 2021 Aug;23(8):1000-1006. doi: 10.1016/j.jmoldx.2021.05.010.
Evaluation of Two Commercial Kits on the Automated ELITe InGenius PCR Platform for Molecular Diagnosis of Toxoplasmosis. Nourrisson C, Moniot M, Poirier P, Sterkers Y. J Mol Diagn. 2021 Jul;23(7):865-871. doi: 10.1016/j.jmoldx.2021.04.004.
Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples. Brenier-Pinchart MP, Varlet-Marie E, Robert-Gangneux F, Filisetti D, Guitard J, Sterkers Y, Yera H, Pelloux H, Bastien P. PLoS One. 2021 Feb 17;16(2):e0246802. doi: 10.1371/journal.pone.0246802.
Molecular diagnosis of toxoplasmosis: recent advances and a look to the future. Robert MG, Brenier-Pinchart MP, Garnaud C, Fricker-Hidalgo H, Pelloux H. Expert Rev Anti Infect Ther. 2021 Dec;19(12):1529-1542. doi: 10.1080/14787210.2021.1941867.
Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification. Roux G, Ravel C, Varlet-Marie E, Jendrowiak R, Bastien P, Sterkers Y. PLoS One. 2019 Sep 27;14(9):e0219276.
Molecular diagnosis of toxoplasmosis at the onset of symptomatic primary infection: A straightforward alternative to serological examinations. Lévêque MF, Chiffré D, Galtier C, Albaba S, Ravel C, Lachaud L, Iemmi A, Flori P, Sterkers Y. Int J Infect Dis. 2019 Feb;79:131-133.
Evolution of Toxoplasma-PCR methods and practices: a French national survey and proposal for technical guidelines. Roux G, Varlet-Marie E, Bastien P, Sterkers Y; French National Reference Center for Toxoplasmosis Network. Int J Parasitol. 2018 Aug;48(9-10):701-707.
A new LAMP-based assay for the molecular diagnosis of toxoplasmosis: comparison with a proficient PCR assay. Varlet-Marie E, Sterkers Y, Perrotte M, Bastien P; ‘Molecular Biology’ working group of the French National Reference Centre for Toxoplasmosis. Int J Parasitol. 2018 May;48(6):457-462.
Evaluation of five automated and one manual method for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid. Yera H, Ménégaut L, Brenier-Pinchart MP, Touafek F, Bastien P, Dalle F; Molecular Biology study group of the French National Reference Centre for Toxoplasmosis. Clin Microbiol Infect. 2018 Oct;24(10):1100.e7-1100.e11.
Evaluation of the Toxoplasma ELITe MGB® real-time PCR assay for the diagnosis of toxoplasmosis. Robert-Gangneux F, Brenier-Pinchart MP, Yera H, Belaz S, Varlet-Marie E, Bastien P; Molecular Biology study group of the French National Reference Center for Toxoplasmosis. J Clin Microbiol. 2017 May;55(5):1369-1376.
Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii. Brenier-Pinchart MP, Capderou E, Bertini RL, Bailly S, Fricker-Hidalgo H, Varlet-Marie E, Murat JB, Sterkers Y, Touafek F, Bastien P, Pelloux H. Diagn Microbiol Infect Dis. 2015 Aug;82(4):289-91.
Molecular diagnosis of toxoplasmosis in immunocompromised patients: a 3-year multicenter retrospective study. Robert-Gangneux F, Sterkers Y, Yera H, Accoceberry I, Menotti J, Cassaing S, Brenier-Pinchart MP, Hennequin C, Delhaes L, Bonhomme J, Villena I, Scherer E, Dalle F, Touafek F, Filisetti D, Varlet-Marie E, Pelloux H, Bastien P. J Clin Microbiol. 2015 May;53(5):1677-84.
Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis. Filisetti D, Sterkers Y, Brenier-Pinchart MP, Cassaing S, Dalle F, Delhaes L, Pelloux H, Touafek F, Varlet-Marie E, Yera H, Candolfi E, Bastien P. J Clin Microbiol. 2015 Jan;53(1):29-34.
Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis. Filisetti D, Yera H, Villard O, Escande B, Wafo E, Houfflin-Debarge V, Delhaes L, Bastien P. J Clin Microbiol. 2015 May;53(5):1719-21.
Freezing and storage at -20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis. Delhaes L, Filisetti D, Brenier-Pinchart MP, Pelloux H, Yéra H, Dalle F, Sterkers Y, Varlet-Marie E, Touafek F, Cassaing S, Bastien P. Diagn Microbiol Infect Dis. 2014 Nov;80(3):197-9.
Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays. Varlet-Marie E, Sterkers Y, Brenier-Pinchart MP, Cassaing S, Dalle F, Delhaes L, Filisetti D, Pelloux H, Touafek F, Yera H, Bastien P. J Clin Microbiol. 2014 Nov;52(11):3952-9.
Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection. Sterkers Y, Pratlong F, Albaba S, Loubersac J, Picot MC, Pretet V, Issert E, Boulot P, Bastien P. J Clin Microbiol. 2012 Dec;50(12):3944-51.
Comparative assessment of a commercial kit and two laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis. Morelle C, Varlet-Marie E, Brenier-Pinchart MP, Cassaing S, Pelloux H, Bastien P, Sterkers Y. J Clin Microbiol. 2012 Dec;50(12):3977-82.
Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood. Sterkers Y, Ribot J, Albaba S, Issert E, Bastien P, Pratlong F. Diagn Microbiol Infect Dis. 2011 Oct;71(2):174-6.
Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens. Sterkers Y, Varlet-Marie E, Cassaing S, Brenier-Pinchart MP, Brun S, Dalle F, Delhaes L, Filisetti D, Pelloux H, Yera H, Bastien P. J Clin Microbiol. 2010 Sep;48(9):3216-22.
Congenital toxoplasmosis in France in 2007: first results from a national surveillance system. Villena I, Ancelle T, Delmas C, Garcia P, Brezin AP, Thulliez P, Wallon M, King L, Goulet V; Toxosurv network and National Reference Centre for Toxoplasmosis. Euro Surveill. 2010 Jun 24;15(25).
Diversity and evolution of methods and practices for the molecular diagnosis of congenital toxoplasmosis in France: a 4-year survey. Sterkers Y, Varlet-Marie E, Marty P, Bastien P; ANOFEL Toxoplasma-PCR Quality Control Group. Clin Microbiol Infect. 2010 Oct;16(10):1594-602.
Multicenter comparative evaluation of five commercial methods for toxoplasma DNA extraction from amniotic fluid. Yera H, Filisetti D, Bastien P, Ancelle T, Thulliez P, Delhaes L. J Clin Microbiol. 2009 Dec;47(12):3881-6.
Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France. Bastien P, Jumas-Bilak E, Varlet-Marie E, Marty P; ANOFEL Toxoplasma-PCR Quality Control Group. Clin Microbiol Infect. 2007 Apr;13(4):430-3.